Thioredoxin Reductase antibody (N-Term)

Catalog No. ABIN190746

The Rabbit Polyclonal anti-Thioredoxin Reductase antibody (ABIN190746) specifically detects Thioredoxin Reductase in WB. The antibody is reactive with Chlamydomonas reinhardtii samples.

Quick Overview for Thioredoxin Reductase antibody (N-Term) (ABIN190746)

Target: Thioredoxin Reductase (TrxR)

Reactivity: Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thioredoxin Reductase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Thioredoxin Reductase Antibody

Binding Specificity: N-Term

Purpose: Anti-NtrC | Thioredoxin reductase (TR/TRxR) (algal)

Cross-Reactivity (Details): Chlamydomonas reinhardtii
Not reactive in: Arabidopsis thaliana

Predicted Reactivity: Chlamydia sp.,Marchantia polymorpha,Nannochloropsis gaditana,Ostreococcus luminarius,Physcomitrium patens,Synechococcus sp.

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7

Application Details

Application Notes: 1 : 500 (WB)

Comment:

antibody will react with long NtrC of Chlamydomonas reinhardtii proteins and shorter NtrA-type rpoteins, which would be distinguished by migration size.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for Thioredoxin Reductase

Target: Thioredoxin Reductase (TrxR)

Alternative Name: Thioredoxin reductase (TR/TRxR) (NtrC)

Background:

Background: Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC 1.8.1.9) which is reducing thoredoxin (Trx). Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis thaliana and Chlmydomonas reinhardtii NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein size of ca. 488 amino acids.

Additional information: Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria

Molecular Weight: 55.3 | 49 kDa (following processing)

Gene ID: 5715808

UniProt: A8HNQ7