MYB antibody (AA 281-294)

Catalog No. ABIN190759

This Goat Polyclonal antibody specifically detects MYB in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for MYB antibody (AA 281-294) (ABIN190759)

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MYB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-MYB Antibody

Binding Specificity: AA 281-294

Purpose: c-Myb (aa281-294)

Sequence: QRHYNDEDPE KEKR

Specificity: This antibody is expected to recognize isoforms 1-7 only (Numbering from NCBI RefSeq). (NP_001123645.1, NP_005366.2, NP_001123644.1, NP_001155128.1, NP_001155129.1, NP_001155130.1, NP_001155131.1)

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QRHYNDEDPEKEKR, from the internal region of the protein sequence according to NP_001123645.1, NP_005366.2, NP_001123644.1, NP_001155128.1, NP_001155129.1, NP_001155130.1, NP_001155131.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 4 μg/mL.

Western Blot: Approx 70 kDa band observed in lysates of cell line MOLT4 and in preliminary testing of Human Bone Marrow and Lymph Node lysate (calculated MW of 72.3 kDa according to NP_005366.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Alternative Name: MYB

Background: C-myb, MYB, v-myb myeloblastosis viral oncogene homolog (avian), c-myb_CDS, efg, Avian myeloblastosis viral (v-myb) oncogene homolog, c-myb protein (140 AA), c-myb10A_CDS, c-myb13A_CDS, c-myb14A_CDS, c-myb8B_CDS, v-myb avian myeloblastosis viral oncogene

Gene ID: 4602, 17863

NCBI Accession: NP_001123645, NP_005366, NP_001123644, NP_001155128, NP_001155129, NP_001155130, NP_001155131

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process