PODXL antibody (C-Term)

Catalog No. ABIN190772

The Goat Polyclonal anti-PODXL antibody is suitable to detect PODXL in samples from Human, Rat and Mouse. It has been validated for WB and ELISA.

Quick Overview for PODXL antibody (C-Term) (ABIN190772)

Target: PODXL (Podocalyxin-Like (PODXL))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PODXL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PODXL Antibody

Binding Specificity: C-Term

Purpose: PODXL

Sequence: DNLTKDDLDE EEDTH

Specificity: This antibody is expected to recognise both reported isoforms (NP_001018121.1 and NP_005388.2).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DNLTKDDLDEEEDTH, from the C Terminus of the protein sequence according to NP_001018121.1 , NP_005388.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 160 kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 58.6 kDa according to NP_001018121.1 and 55.4 kDa according to NP_005388.2). The observed molecular weight corresponds to earlier findings in literature wit

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Alternative Name: PODXL

Background: PODXL, podocalyxin-like, Gp200, MGC138240, PCLP

Gene ID: 5420, 27205, 192181

NCBI Accession: NP_001018121, NP_005388

Pathways: Tube Formation