GABPB2 antibody (Internal Region)

Catalog No. ABIN190778

The Goat Polyclonal anti-GABPB2 antibody has been validated for WB, ELISA, IHC, IF and FACS. It is suitable to detect GABPB2 in samples from Human.

Quick Overview for GABPB2 antibody (Internal Region) (ABIN190778)

Target: GABPB2 (GA-Binding Protein Subunit beta-2 (GABPB2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GABPB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-GABPB2 Antibody

Binding Specificity: Internal Region

Purpose: GABPB2

Sequence: SSENSSKATD ETG

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SSENSSKATDETG, from the internal region of the protein sequence according to NP_005245.2, NP_002032.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 45+38 kDa bands observed in lysates of cell line HeLa (calculated MW of 42.5 kDa according to NP_005245.2, NP_001307844.1 and of 38.1 kDa according to NP_002032.2). An additional band of unknown identity was also consistently observed

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GABPB2

Target: GABPB2 (GA-Binding Protein Subunit beta-2 (GABPB2))

Alternative Name: GABPB2

Background: GABPB2, GA binding protein transcription factor, beta subunit 2, BABPB2, E4TF1, E4TF1-47, E4TF1-53, E4TF1B, GABPB, GABPB1, NRF2B1, NRF2B2, GA binding protein transcription factor, beta subunit 1, 53 kDa, GA binding protein transcription factor, beta subuni

Gene ID: 2553

NCBI Accession: NP_005245, NP_002032, NP_001307844