GAB2 antibody (Internal Region)

Catalog No. ABIN190799

The Goat Polyclonal anti-GAB2 antibody has been validated for WB and ELISA. It is suitable to detect GAB2 in samples from Human.

Quick Overview for GAB2 antibody (Internal Region) (ABIN190799)

Target: GAB2 (GRB2-Associated Binding Protein 2 (GAB2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GAB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GAB2 Antibody

Binding Specificity: Internal Region

Purpose: GAB2

Sequence: QVDKEKTQAL QN

Specificity: This antibody is expected to recognize both reported isoforms (NP_536739.1, NP_036428.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QVDKEKTQALQN, from the internal region (near the C Terminus) of the protein sequence according to NP_536739.1, NP_036428.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in lysates of cell line K562 (calculated MW of 74.5 kDa according to NP_536739.1). Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GAB2

Target: GAB2 (GRB2-Associated Binding Protein 2 (GAB2))

Alternative Name: GAB2

Background: GAB2, GRB2-associated binding protein 2, KIAA0571, Grb2-associated binder 2

Gene ID: 9846, 14389, 84477

NCBI Accession: NP_536739, NP_036428

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, BCR Signaling, Warburg Effect