TIA1 antibody (Internal Region)

Catalog No. ABIN190808

The Goat Polyclonal anti-TIA1 antibody has been validated for WB, IF, IHC and ELISA. It is suitable to detect TIA1 in samples from Human.

Quick Overview for TIA1 antibody (Internal Region) (ABIN190808)

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TIA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-TIA1 Antibody

Binding Specificity: Internal Region

Purpose: TIA1

Sequence: PKSTYESNTK Q

Specificity: This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PKSTYESNTKQ, from the internal region of the protein sequence according to NP_071320.1, NP_071505.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thyroid. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8 kDa according to NP_071320.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. An additional band at 80 kDa and a very strong 26 kDa is a

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TIA1

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Alternative Name: TIA1

Background: TIA1, TIA1 cytotoxic granule-associated RNA binding protein , TIA1 cytotoxic granule-associated RNA-binding protein, TIA1 protein, cytotoxic granule-associated RNA-binding protein, p40-TIA-1 (containing p15-TIA-1)

Gene ID: 7072, 21841, 312510

NCBI Accession: NP_071320, NP_071505