CYLD antibody (Internal Region)

Catalog No. ABIN190823

The Goat Polyclonal anti-CYLD antibody is suitable to detect CYLD in samples from Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for CYLD antibody (Internal Region) (ABIN190823)

Target: CYLD (Cylindromatosis (Turban Tumor Syndrome) (CYLD))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CYLD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CYLD Antibody

Binding Specificity: Internal Region

Purpose: Cyld (mouse)

Sequence: DSQQSKSKNP WYIDE

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DSQQSKSKNPWYIDE, from the internal region of the protein sequence according to NP_775545.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Mouse Brain. Recommended concentration: 5 μg/mL.

Western Blot: Approx 125 kDa band observed in Rat Brain lysates (calculated MW of 107 kDa according to mouse NP_775545.1). Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CYLD

Target: CYLD (Cylindromatosis (Turban Tumor Syndrome) (CYLD))

Alternative Name: Cyld

Background: CYLD, cylindromatosis (turban tumor syndrome) , 2010013M14Rik, 2900009M21Rik, C130039D01Rik, CDMT, CYLD1, EAC, mKIAA0849, ubiquitin carboxyl-terminal hydrolase CYLD

Gene ID: 74256, 312937

NCBI Accession: NP_775545

Pathways: Apoptosis, Activation of Innate immune Response