Liver Arginase antibody (C-Term)

Catalog No. ABIN190828

This Goat Polyclonal antibody specifically detects Liver Arginase in WB and ELISA. It exhibits reactivity toward Rat and Mouse and has been mentioned in 2+ publications.

Quick Overview for Liver Arginase antibody (C-Term) (ABIN190828)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivity: Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Liver Arginase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Liver Arginase Antibody

Binding Specificity: C-Term

Purpose: Arginase, type 1 / arg1(rat)

Sequence: NHKPETDYLK PPK

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-NHKPETDYLKPPK

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 37 kDa band observed in Mouse and Rat Liver lysates (calculated MW of 35 kDa according to Rat NP_058830.2 and 34.8 kDa according to Mouse NP_031508.1). Recommended concentration: 0.003-0.01 μg/mL. Primary incubation 1 hour at room tempe

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Liver Arginase antibody (ABIN190828)

Sherman, Gutman, Chapnik, Meylan, le Coutre, Froy: "All-trans retinoic acid modifies the expression of clock and disease marker genes." in: The Journal of nutritional biochemistry, Vol. 23, Issue 3, pp. 209-17, (2012) (PubMed).

Sherman, Gutman, Chapnik, Meylan, le Coutre, Froy: "Caffeine alters circadian rhythms and expression of disease and metabolic markers." in: The international journal of biochemistry & cell biology, Vol. 43, Issue 5, pp. 829-38, (2011) (PubMed).

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: Arg1

Background: Arg1, arginase 1, AI type I arginase, arginase 1 liver

Gene ID: 11846, 29221

NCBI Accession: NP_058830

Pathways: Cellular Response to Molecule of Bacterial Origin