Latexin antibody (C-Term)

Catalog No. ABIN190839

This anti-Latexin antibody is a Goat Polyclonal antibody detecting Latexin in WB, ELISA, IHC and IF. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Latexin antibody (C-Term) (ABIN190839)

Target: Latexin (LXN)

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Latexin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-Latexin Antibody

Binding Specificity: C-Term

Purpose: Latexin

Sequence: QYGTKVKHNS RLPKE

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QYGTKVKHNSRLPKE, from the C Terminus of the protein sequence according to NP_064554.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: This antibody has been successfully used in IHC-P for mouse bones as described in the following paper: Kadouchi et al, Biochem Biophys Res Commun. 2009 Jan 16,378(3):600-4, PMID: 19059214.

Western Blot: Approx 28 kDa band observed in lysates of Human Brain (Cerebellum), Mouse Brain and Rat Brain (calculated MW of 25.8 kDa according to NP_064554.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Latexin antibody (ABIN190839)

Mitsunaga, Kikuchi, Wada, Furukawa: "Latexin regulates the abundance of multiple cellular proteins in hematopoietic stem cells." in: Journal of cellular physiology, Vol. 227, Issue 3, pp. 1138-47, (2012) (PubMed).

Kadouchi, Sakamoto, Tangjiao, Murakami, Kobayashi, Hoshino, Yamaguchi: "Latexin is involved in bone morphogenetic protein-2-induced chondrocyte differentiation." in: Biochemical and biophysical research communications, Vol. 378, Issue 3, pp. 600-4, (2008) (PubMed).

Target Details for Latexin

Target: Latexin (LXN)

Alternative Name: LXN

Background: Latexin, latexin protein, LXN, ECI, TCI

Gene ID: 56925, 17035, 59073

NCBI Accession: NP_064554