NPY2R antibody (Internal Region)

Catalog No. ABIN190857

This anti-NPY2R antibody is a Goat Polyclonal antibody detecting NPY2R in WB and ELISA. Suitable for Rat.

Quick Overview for NPY2R antibody (Internal Region) (ABIN190857)

Target: NPY2R (Neuropeptide Y Receptor Y2 (NPY2R))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This NPY2R antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-NPY2R Antibody

Binding Specificity: Internal Region

Purpose: Npy2r (mouse)

Sequence: KAKKNLEVKK NN

Specificity: Pease note this antibody was designed using the Mouse sequence NP_032757.1, which differs by 1 amino acid from the Human sequence NP_000901.1.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KAKKNLEVKKNN, from the internal region of the protein sequence according to NP_032757.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40+45 kDa band observed in Rat Brain lysates (calculated MW of 42.6 kDa according to NP_032757.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunoprecipitation: A customer successfully used this product for IP of Human Y2R, but the Human Y2R could not be detected in WB by this antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NPY2R

Target: NPY2R (Neuropeptide Y Receptor Y2 (NPY2R))

Alternative Name: Npy2r

Background: Npy2r, neuropeptide Y receptor Y2, MGC124241, MGC124242, MGC124244, NPY-Y2 receptor

Gene ID: 4887, 18167, 66024

NCBI Accession: NP_032757

Pathways: cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour