TMED10 antibody (C-Term)

Catalog No. ABIN190858

This Goat Polyclonal antibody specifically detects TMED10 in ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for TMED10 antibody (C-Term) (ABIN190858)

Target: TMED10 (Transmembrane Emp24-Like Trafficking Protein 10 (TMED10))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TMED10 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-TMED10 Antibody

Binding Specificity: C-Term

Purpose: TMP21 / TMED10

Sequence: YLRRFFKAKK LIE

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YLRRFFKAKKLIE, from the C Terminus of the protein sequence according to NP_006818.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 75 kDa band in Rat Pancreas lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0kD

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

Immunofluorescence:Expression of the protein seen in the Golgi and cytoplasm of Glioblastoma U251 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentra

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TMED10

Target: TMED10 (Transmembrane Emp24-Like Trafficking Protein 10 (TMED10))

Alternative Name: TMED10

Background: TMED10, transmembrane emp24-like trafficking protein 10 (yeast), P24(DELTA), S31I125, S31III125, TMP21, Tmp-21-I , 21 kDa transmembrane trafficking protein, transmembrane emp24 domain-containing protein 10

Gene ID: 10972, 68581, 84599

NCBI Accession: NP_006818