IER5 antibody (Internal Region)

Catalog No. ABIN190870

This Goat Polyclonal antibody specifically detects IER5 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for IER5 antibody (Internal Region) (ABIN190870)

Target: IER5 (Immediate Early Response 5 (IER5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IER5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IER5 Antibody

Binding Specificity: Internal Region

Purpose: IER5

Sequence: QPPSGGEDDD AEE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QPPSGGEDDDAEE, from the internal region of the protein sequence according to NP_057629.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Customer's experiments gave a band at approx 45 kDa in lysates of transfected H1299 overexpressing IER5 but not when transfected with empty vector (calculated MW of 33.7 kDa according to NP_057629.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IER5

Target: IER5 (Immediate Early Response 5 (IER5))

Alternative Name: IER5

Background: IER5, immediate early response 5, MGC102760, SBBI48

Gene ID: 51278

NCBI Accession: NP_057629