TRAF2 antibody (C-Term)

Catalog No. ABIN190911

The Goat Polyclonal anti-TRAF2 antibody (ABIN190911) specifically detects TRAF2 in WB, IHC, ELISA, IF and FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for TRAF2 antibody (C-Term) (ABIN190911)

Target: TRAF2 (TNF Receptor-Associated Factor 2 (TRAF2))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This TRAF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-TRAF2 Antibody

Binding Specificity: C-Term

Purpose: TRAF2

Sequence: KMEAKNSYVR DD

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KMEAKNSYVRDD, from the C Terminus of the protein sequence according to NP_066961.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Pancreas, Kidney and Placenta. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Ovary and Mouse Testis lysates and in lysates of cell lines HepG2 and Jurkat. Preliminary testing also showed a band at approx 50 kDa in U937, NIH3T3 and HEK293 cell lysates (calculated MW of 55.9 kDa, acco

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulums of HeLa and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended co

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRAF2

Target: TRAF2 (TNF Receptor-Associated Factor 2 (TRAF2))

Alternative Name: TRAF2

Background: TRAF2, TNF receptor-associated factor 2 , MGC:45012, TRAP, TRAP3 , OTTHUMP00000064745, tumor necrosis factor type 2 receptor associated protein 3

Gene ID: 7186, 22030

NCBI Accession: NP_066961

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response, S100 Proteins