SUPT16H antibody (Internal Region)

Catalog No. ABIN190913

The Goat Polyclonal anti-SUPT16H antibody is suitable to detect SUPT16H in samples from Human. It has been validated for ELISA and WB.

Quick Overview for SUPT16H antibody (Internal Region) (ABIN190913)

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SUPT16H antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Verified

Product Details anti-SUPT16H Antibody

Binding Specificity: Internal Region

Purpose: FACT / SUPT16H

Sequence: DLTNKEGKKP EEK

Cross-Reactivity: Cow, Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DLTNKEGKKPEEK, from the internal region of the protein sequence according to NP_009123.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 150 kDa band observed in lysates of cell line HeLa (calculated MW of 120 kDa according to NP_009123.1). Recommended concentration: 0.1-0.3 μg/mL. An additional band of unknown identity was also consistently observed at 30 kDa. This band

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SUPT16H

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Alternative Name: SUPT16H

Background: SUPT16H, FACT, suppressor of Ty 16 homolog (S. cerevisiae), HGNC:11465, CDC68, FACTP140, FLJ10857, FLJ14010, SPT16/CDC68, chromatin-specific transcription elongation factor large subunit, FLJ34357, facilitates chromatin remodeling 140 kDa subunit

Gene ID: 11198

NCBI Accession: NP_009123

Pathways: Chromatin Binding