PIWIL1 antibody (Internal Region)

Catalog No. ABIN190916

The Goat Polyclonal anti-PIWIL1 antibody is suitable to detect PIWIL1 in samples from Human. It has been validated for IHC, ELISA, IF and FACS.

Quick Overview for PIWIL1 antibody (Internal Region) (ABIN190916)

Target: PIWIL1 (Piwi-Like 1 (PIWIL1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PIWIL1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PIWIL1 Antibody

Binding Specificity: Internal Region

Purpose: PIWI / HIWI

Sequence: SNRKDKYDAI KKY

Specificity: This antibody is expected to recognise both reported isoforms (NP_004755.2, NP_001177900.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SNRKDKYDAIKKY, from the internal region of the protein sequence according to NP_004755.2, NP_001177900.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration: 4-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PIWIL1

Target: PIWIL1 (Piwi-Like 1 (PIWIL1))

Alternative Name: PIWIL1

Background: PIWIL1, HIWI, PIWI, piwi-like 1 (Drosophila), piwi (Drosophila)-like 1, homolog of Drosophila piwi, MIWI, piwi homolog, piwi-like 1

Gene ID: 9271, 57749

NCBI Accession: NP_004755, NP_001177900