HSP70 antibody

Catalog No. ABIN191987

This Mouse Monoclonal antibody specifically detects HSP70 in WB, IP, IHC (fro) and EIA. It exhibits reactivity toward Geodia cydonium.

Quick Overview for HSP70 antibody (ABIN191987)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Geodia cydonium

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: IVF5

Product Details anti-HSP70 Antibody

Specificity: This antibody detects HSP70.

Characteristics: Synonyms: Heat Shock Protein 70

Purification: Protein G chromatography

Immunogen: HSP70 from Geodia cydonium

Isotype: IgG1

Application Details

Application Notes: ELISA: 1: 5,000. Western blot: 1: 500. Immunoprecipitation: 1: 50. Immunohistochemistry: 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in aqua bidest to 1 mg/mL.

Buffer: 50 mM TRIS pH 7.4

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2 - 8 °C and reconstituted at -20 °C. Avoid repeated freezing andthawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: Hsp70

Background: Heat shock proteins (HSPs) or stress response proteins (SRPs) are synthesized in variety of environmental and pathophysiological stressful conditions. Many HSPs are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation, and secretion. HSP70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides, and other unknown proteins. Also, HSP70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs, and other damaging conditions. Heat shock proteins are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. Eukaryotic cells contain an HSP70 multigene that differ in their intracellular location and regulation. Members of the HSP70 family play a major role in the folding, unfolding and translocation of polypeptides as well as in the assembly and disassembly of oligomeric protein complexes, as well as in the immune response. HSP72 was found to increase dramatically in the brains of Alzheimer's disease patients, and was localized exclusively in neuritic plaques and neurofibrillary tangles. HSP70 concentrates in nuclei during heat shock and returns to the cytoplasm when the shock is removed.Synonyms: Heat Shock Protein 70

UniProt: Q24952