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IgE antibody (FITC)

This anti-IgE antibody is a Mouse Monoclonal antibody detecting IgE in FACS. Suitable for Human. This Primary Antibody has been cited in 1 publication.
Catalog No. ABIN192255

Quick Overview for IgE antibody (FITC) (ABIN192255)

Target

See all IgE Antibodies
IgE

Reactivity

  • 24
  • 3
  • 2
  • 1
Human

Host

  • 17
  • 10
  • 2
  • 1
Mouse

Clonality

  • 22
  • 7
  • 1
Monoclonal

Conjugate

  • 22
  • 3
  • 2
  • 2
  • 1
This IgE antibody is conjugated to FITC

Application

  • 19
  • 9
  • 8
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS)

Clone

BE5
  • Purpose

    Anti-Hu IgE FITC

    Specificity

    The antibody BE5 reacts with human IgE, it recognizes an epitope different from the ones recognized by 4G7.325 and 4H10 antibodies to IgE.

    Cross-Reactivity (Details)

    Human

    Purification

    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

    Immunogen

    Purified human IgE.

    Isotype

    IgG1
  • Application Notes

    Flow cytometry: Recommended dilution: 5 μg/mL.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.1 mg/mL

    Buffer

    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze.
    Avoid prolonged exposure to light.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Kaulfürst-Soboll, Mertens, Brehler, von Schaewen: "Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis." in: PLoS ONE, Vol. 6, Issue 3, pp. e17800, (2011) (PubMed).

  • Target

    IgE

    Background

    Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E

    Molecular Weight

    180 kDa
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