CEACAM5 antibody

Catalog No. ABIN192374

The Mouse Monoclonal anti-CEACAM5 antibody has been validated for FACS, IHC (p), IHC (fro) and IP. It is suitable to detect CEACAM5 in samples from Human.

Quick Overview for CEACAM5 antibody (ABIN192374)

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Clone: CB30

Product Details anti-CEACAM5 Antibody

Purpose: Anti-Hu CD66e Purified

Specificity: The mouse monoclonal antibody CB30 recognizes CD66e (CEA, 180-200 kDa), an extracellular cell surface-bound carcinoembryonic antigen mainly expressed on epithelial cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human carcinoembryonic antigen (CEA, CEACAM5)

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Staining technique: standard ABC technique (DAB+), recommended dilution: 10 μg/mL (1:100), positive tissue: adenocarcinoma of colon, pretreatment: 0,1 % pepsin in 0,1 M HCl for 30 min at room temperature.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CEACAM5

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Alternative Name: CD66e

Background: Carcinoembryonic antigen related cell adhesion mol,The CD66e (CEA, 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting.,CEACAM5, CEA

Gene ID: 1048

UniProt: P06731