Pyruvate Kinase, Muscle (PKM) (AA 481-531) antibody

Catalog No. ABIN1924307

The Rabbit Polyclonal anti-not_set antibody has been validated for WB, IHC, IHC (p), IF, IP and PLA. It is suitable to detect not_set in samples from Human, Mouse, Rat, Xenopus laevis, Chicken and Zebrafish (Danio rerio).

Quick Overview for Pyruvate Kinase, Muscle (PKM) (AA 481-531) antibody (ABIN1924307)

Target: Pyruvate Kinase, Muscle (PKM)

Reactivity: Human, Mouse, Rat, Xenopus laevis, Chicken, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Proximity Ligation Assay (PLA)

Product Details

Binding Specificity: AA 481-531

Specificity: Region between residue 481 and 531 of human Pyruvate Kinase Isozymes M1/M2 using the numbering given in entry NP_002645.3 (GeneID 5315).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:500 - 1:2000), IHC, IHC-P (1:1000 - 1:5000), IP (2 - 10 μg/mg lysate), PLA (1:1000 - 1:20000), WB (1:2000 - 1:10000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Linitis Plastica Stomach Cancer, Osteosarcoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details

Target: Pyruvate Kinase, Muscle (PKM)

Alternative Name: PKM / Pyruvate Kinase, Muscle

Background: Name/Gene ID: PKM

Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1

Gene ID: 5315

Pathways: Warburg Effect