AMPK alpha antibody (N-Term)

Catalog No. ABIN1926452

The Mouse Monoclonal anti-AMPK alpha antibody has been validated for WB, IHC, ELISA, IF, IHC (p), IP and IHC (fro). It is suitable to detect AMPK alpha in samples from Human, Mouse, Rat and Drosophila melanogaster.

Quick Overview for AMPK alpha antibody (N-Term) (ABIN1926452)

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Reactivity: Human, Mouse, Rat, Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This AMPK alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-AMPK alpha Antibody

Binding Specificity: N-Term

Specificity: Recognizes both the alpha 1 and the alpha 2 isoforms of AMP-activated protein kinase (AMPK). Species cross-reactivity: Drosophila, human, mouse, rat.

Purification: Protein G purified

Immunogen: Synthetic peptide corresponding to 29 amino acid within the N-terminal catalytic domain of Drosophila AMPK alpha.

Type of Immunogen: Synthetic peptide

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-Fr, IHC-P (10 μg/mL), IP, WB (1:100 - 1:1000)

Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:100-1:1000 ~63kD in rat brain cell lysates. Immunohistochemistry: Frozen or Paraffin.

Comment:

Target Species of Antibody: Drosophila

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for AMPK alpha

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Alternative Name: PRKAA1 + PRKAA2

Background: Name/Gene ID: PRKAA1 / PRKAA2

Synonyms: PRKAA1 / PRKAA2, AMPK1/2, AMPK1/AMPK2

This antibody detects: PRKAA1 + PRKAA2

Pathways: Warburg Effect