EGFR antibody

Catalog No. ABIN1944913

This Rabbit Polyclonal antibody specifically detects EGFR in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 5+ publications.

Quick Overview for EGFR antibody (ABIN1944913)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EGFR Antibody

Isotype: IgG

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C,-20 °C

References for anti-EGFR antibody (ABIN1944913)

Reiter, Threadgill, Eley, Strunk, Danielsen, Sinclair, Pearsall, Green, Yee, Lampland, Balasubramaniam, Crossley, Magnuson, James, Maihle: "Comparative genomic sequence analysis and isolation of human and mouse alternative EGFR transcripts encoding truncated receptor isoforms." in: Genomics, Vol. 71, Issue 1, pp. 1-20, (2001) (PubMed).

Ilekis, Gariti, Niederberger, Scoccia: "Expression of a truncated epidermal growth factor receptor-like protein (TEGFR) in ovarian cancer." in: Gynecologic oncology, Vol. 65, Issue 1, pp. 36-41, (1997) (PubMed).

Reiter, Maihle: "A 1.8 kb alternative transcript from the human epidermal growth factor receptor gene encodes a truncated form of the receptor." in: Nucleic acids research, Vol. 24, Issue 20, pp. 4050-6, (1996) (PubMed).

Ilekis, Stark, Scoccia: "Possible role of variant RNA transcripts in the regulation of epidermal growth factor receptor expression in human placenta." in: Molecular reproduction and development, Vol. 41, Issue 2, pp. 149-56, (1995) (PubMed).

Ullrich, Coussens, Hayflick, Dull, Gray, Tam, Lee, Yarden, Libermann, Schlessinger: "Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells." in: Nature, Vol. 309, Issue 5967, pp. 418-25, (1984) (PubMed).

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.

Molecular Weight: 134277 Da

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins