TUBA1B antibody (C-Term)

Catalog No. ABIN1945559

The Mouse Monoclonal anti-TUBA1B antibody has been validated for WB, ELISA, IF, IHC and IHC (p). It is suitable to detect TUBA1B in samples from Human, Mouse, Rat, Chicken and Cow.

Quick Overview for TUBA1B antibody (C-Term) (ABIN1945559)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivity: Human, Mouse, Rat, Chicken, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBA1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 17H11-F10

Product Details anti-TUBA1B Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Anti-Tubulin Loading Control Antibody was purified Protein A chromatography. This Loading Control antibody is directed against alpha tubulin. A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100 % homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

Purification: Protein A purified

Sterility: Sterile filtered

Immunogen: Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:300000), IF (0.1 μg/mL), IHC, IHC-P (5 μg/mL), WB (1:1000)

Usage: Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kD in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Alternative Name: TUBA1B

Background: Name/Gene ID: TUBA1B

Synonyms: TUBA1B, Alpha-tubulin ubiquitous, Alpha tubulin, K-ALPHA-1, Tubulin, alpha 1b, Tubulin alpha-ubiquitous chain, Tubulin, alpha, ubiquitous, Tubulin K-alpha-1, Tubulin alpha chain, Tubulin alpha-1B chain

Gene ID: 10376

Pathways: Microtubule Dynamics, M Phase