SEMA3B antibody (AA 100-200)

Catalog No. ABIN1951824

The Rabbit Polyclonal anti-SEMA3B antibody (ABIN1951824) specifically detects SEMA3B in WB, ICC and IF. The antibody is reactive with Human, Mouse, Rat, Cow, Dog and Primate samples.

Quick Overview for SEMA3B antibody (AA 100-200) (ABIN1951824)

Target: SEMA3B (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B))

Reactivity: Human, Mouse, Rat, Cow, Dog, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEMA3B antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-SEMA3B Antibody

Binding Specificity: AA 100-200

Specificity: Immunohistochemistry-Paraffin Embedded Sections
Note: SEMA3B protein is secreted and accumulates in the endoplasmic reticulum.
Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool
slides on bench-top for 30 minutes.
Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 µL DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

Purification: Aff - Purified

Immunogen: A synthetic peptide made to an internal portion of the human SEMA 3B protein sequence (AA100-200).

Isotype: IgG

Application Details

Application Notes: Western Blot 1:5000 , Immunohistochemistry: 1:100 (paraffin-embedded tissue), Immunocytochemistry/Immunofluorescence 1:40 Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Tris-glycine, 150 mM NaCL and 0.05% Sodium Azide as a preservative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Repeated freeze/thaw cycles

Storage: -20 °C

Storage Comment: Store frozen. Aliquot as undiluted antisera and immediately place at -20°C. Antisera may have become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening. Stable for at least 6 months at -20°C. Compromise the integrity of the antiserum.

Target Details for SEMA3B

Target: SEMA3B (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B))

Alternative Name: SEMA3B