This Mouse Monoclonal antibody specifically detects CASR in WB, ELISA, IF, ICC, IP and RIA. It exhibits reactivity toward Human, Mouse, Rat and Rabbit.
Catalog No. ABIN1951849
Quick Overview for CASR antibody (N-Term) (ABIN1951849)
This Osteopontin (1B20) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In Western blot multiple bands can be seen due to glycosylation and phosphorylation of the protein. Image: Western Blot of Calcium Sensing Receptor in transfected 293 cell lysate. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.
Purification
Purified
Immunogen
N-terminal, extracellular region of , human Calcium Sensing Receptor.
Western Blot 1:50-1:200, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200, Immunoprecipitation 5-10 µg Radioimmunoassay. Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
Liquid with Tris-glycine 150mM NaCl pH 7.5.
Handling Advice
Repeated freeze/thaw cycles , compromise the integrity of the antiserum.
Storage
-20 °C
Storage Comment
Store frozen. Aliquot as undiluted antisera and immediately place at -20°C. Antisera may have become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening. Stable for at least 6 months at -20°C.