GLB1 antibody (HRP)

Catalog No. ABIN1952606

The Rabbit Polyclonal anti-GLB1 antibody has been validated for WB, IHC, ELISA, IP, IF and DB. It is suitable to detect GLB1 in samples from E. coli.

Quick Overview for GLB1 antibody (HRP) (ABIN1952606)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLB1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB)

Product Details anti-GLB1 Antibody (HRP)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, and anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli]. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.

Purification: Ion exchange chromatography

Immunogen: Beta Galactosidase (E. coli).

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: DB, ELISA (1:10000 - 1:50000), IF, IHC (1:500 - 1:2000), IP, WB (1:2000 - 1:5000)

Usage: Anti-Beta Galactosidase is suitable for immunoblotting (western or dot blot), ELISA, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4 % paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus. A 1:1 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4 % paraformaldehyde and cryostat-cut (35 mu m) to produce free-floating sections. A 1:5000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy. A 1:5000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. Although a wide range of conditions was reported to be effective, a 1:10000 dilution was noted to show no background and to be suitable for double labeling experiments. Optimal titers for other applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: E. coli

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Target Details for GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: Beta Galactosidase

Pathways: Glycosaminoglycan Metabolic Process