CXCR3 antibody

Catalog No. ABIN1967162

This Mouse Monoclonal antibody specifically detects CXCR3 in FACS, IHC, Neut and Func. It exhibits reactivity toward Human.

Quick Overview for CXCR3 antibody (ABIN1967162)

Target: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CXCR3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut), Functional Studies (Func)

Clone: 2AR1

Product Details anti-CXCR3 Antibody

Specificity: Recognizes human CXCR3. Reacts specifically with CXCR3 transfectants and not the parental cell line using FACS analysis. Does not cross-react with CXCR1, CXCR2, CXCR4 or transfectants.

Purification: Protein G purified

Immunogen: CXCR3 transfected NS0 cells.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (5 - 10 μg/mL), Func, IHC (10 μg/mL), Neut

Usage: Suitable for use in Neutralization, Flow Cytometry and Immunohistochemistry. Neutralization (ND50): The exact concentration required to neutralize human cell surface CXCR3 activity is dependent on the ligand concentration as well as the number of CXCR3 receptors present on the cell surface. The Neutralization Dose50 (ND50) is defined as the concentration of antibody required to yield one half maximal inhibition of the cell surface CXCR3 mediated rhl-TAC response on a responsive cell line, at a specific rhl-TAC concentration. The ND50 for this lot was determined to be ~1-6 μg/mL in the presence of 7 ng/mL of rhl-TAC, using the hCXCR3 transfected BaF/3 cells in a chemotaxis assay. Flow Cytometry: 5-10 μg/mL, Add 10 μL to 1-2.5x10^5 cells in total reaction volume 200 μL. Visualize binding by adding 10 μL of 25 μg/mL IgG (FITC) goat anti-mouse. Immunohistochemistry: 10 μg/mL detected CXCR3 in human tonsil. Cells were fixed in PBS, 4 % paraformaldehyde and blocked with PBS, 10 % normal donkey serum, 0.1 % Triton X-100, 1 % BSA. Cells were washed with PBS, BSA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

Target Details for CXCR3

Target: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Alternative Name: CXCR3

Background: Name/Gene ID: CXCR3
Subfamily: Chemokine
Family: GPCR

Synonyms: CXCR3, C-x-c chemokine receptor 3, CD183 antigen, CKR-L2, CXCR-3, CMKAR3, CD182, CD183, Cxcr3a, GPR9, IP-10 receptor, IP10-R, Mig receptor, MigR, Mig-R, Chemokine (C-X-C) receptor 3, CXC-R3, Cxcr3b, G protein-coupled receptor 9, IP10 receptor

Gene ID: 2833

UniProt: P49682