MHC Class II antibody

Catalog No. ABIN1967973

The Rat Monoclonal anti-MHC Class II antibody (Clone M5-114) (ABIN1967973) specifically detects MHC Class II in FACS, WB, IHC, IP, IHC (fro), Func, IHC (p), BR and Neut. The antibody is reactive with Mouse samples.

Quick Overview for MHC Class II antibody (ABIN1967973)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MHC Class II antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR), Neutralization (Neut)

Clone: M5-114

Product Details anti-MHC Class II Antibody

Specificity: Monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

Purification: Protein G purified

Sterility: Sterile filtered

Immunogen: Activated C57BL/6 mouse spleen cells.

Type of Immunogen: Cells

Isotype: IgG2b

Application Details

Application Notes: Approved: Block, Flo, Func, IHC, IHC-Fr, IHC-P, IP, Neut, WB

Usage: Flow Cytometry: Recommended dilution - 2 μg/mL. Functional Application: Blocking of T cell proliferative responses. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, sterile filtered.

Preservative: Azide free

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Alternative Name: MHC Class II