14-3-3 zeta antibody (pSer58)

Catalog No. ABIN196916

The Rabbit Polyclonal anti-14-3-3 zeta antibody is suitable to detect 14-3-3 zeta in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for 14-3-3 zeta antibody (pSer58) (ABIN196916)

Target: 14-3-3 zeta (YWHAZ)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 zeta antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-14-3-3 zeta Antibody

Binding Specificity: pSer58

Specificity: This antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at Serine 58.

Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide.

Immunogen: Peptide Sequence around the phosphorylation site of Serine 58 (R-S-Sp-W-R) derived from Human 14-3-3 zeta.

Application Details

Application Notes: Suitable for use in Suitable for use in Western blot (1: 500-1: 1000) andImmunohistochemistry on paraffin-embedded sections (1: 50-1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: 14-3-3 Protein zeta/delta

Background: Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ

Gene ID: 7534

NCBI Accession: NP_001129171

UniProt: P63104

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location