ERK1/2 antibody (pThr187, pTyr204)

Catalog No. ABIN197000

The Rabbit Polyclonal anti-ERK1/2 antibody has been validated for WB, IHC (p) and IF. It is suitable to detect ERK1/2 in samples from Human, Mouse and Rat.

Quick Overview for ERK1/2 antibody (pThr187, pTyr204) (ABIN197000)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-ERK1/2 Antibody

Binding Specificity: pThr187, pTyr204

Specificity: This antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at Tyrosine 204.

Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human p44/42 MAP Kinase around the phosphorylation site of Tyrosine 204 (T-E-Yp-V-A).

Application Details

Application Notes: Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/100. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: 1. TETE HANNKEN, et al. (2000) Am Soc Nephrol 11: 1387-13972. Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 1623. Jingui Yu, et al. (2005) Anesth Analg 101: 315-3214. Hironobu Ihn et al. (2000) Immunology 165: 2149-2155

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: ERK1 / ERK2

Background: Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.Synonyms: ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3, P42/P44-MAPK