Ephrin B2 antibody (Tyr316)

Catalog No. ABIN197416

This Rabbit Polyclonal antibody specifically detects Ephrin B2 in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for Ephrin B2 antibody (Tyr316) (ABIN197416)

Target: Ephrin B2 (EFNB2)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Ephrin B2 Antibody

Binding Specificity: Tyr316

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Ephrin-B2 antibody AP02707PU detects endogenous levels of total Ephrin-B2 protein.

Purification: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Ephrin-B2 around the phosphorylation site of tyrosine 316 (P-V-Yp-I-V).

Application Details

Application Notes: Suitable for use in in Western blot (1: 500-1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Ephrin B2

Target: Ephrin B2 (EFNB2)

Alternative Name: Ephrin-B2

Background: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP)and also interacts with PRKCABP and GRIP1. This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. Ephrin-B2 also binds EphA4, EphB1, EphB2, EphB3 and EphB4. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.Synonyms: EFNB2, EPH-related receptor tyrosine kinase ligand 5, EPLG5, HTK ligand, HTK-L, HTKL, LERK-5, LERK5

Gene ID: 1948

NCBI Accession: NP_004084

UniProt: P52799

Pathways: RTK Signaling, Regulation of Muscle Cell Differentiation