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IRS1 antibody (Insulin Receptor Substrate 1) (Ser636) Primary Antibody

IRS1 Reactivity: Human, Mouse, Rat IF, IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN197467
$350.90
Plus shipping costs $45.00
50 μL ABIN197467
50 μL ABIN197467
local_shipping Shipping to: United States
Available
  • Target
    IRS1
    Binding Specificity
    • 31
    • 30
    • 25
    • 21
    • 20
    • 20
    • 19
    • 17
    • 17
    • 16
    • 16
    • 16
    • 16
    • 15
    • 15
    • 13
    • 11
    • 8
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ser636
    Reactivity
    • 377
    • 308
    • 283
    • 34
    • 27
    • 19
    • 18
    • 18
    • 16
    • 16
    • 13
    • 10
    • 2
    Human, Mouse, Rat
    Host
    • 381
    • 14
    Rabbit
    Clonality
    • 373
    • 22
    Polyclonal
    Conjugate
    • 157
    • 23
    • 19
    • 16
    • 16
    • 16
    • 16
    • 16
    • 15
    • 15
    • 14
    • 14
    • 14
    • 14
    • 14
    • 11
    • 2
    • 1
    • 1
    • 1
    This IRS1 antibody is un-conjugated
    Application
    • 178
    • 146
    • 139
    • 106
    • 105
    • 93
    • 67
    • 53
    • 24
    • 24
    • 19
    • 11
    • 7
    • 2
    • 2
    Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Specificity
    This antibody AP02732PU detects endogenous levels of total IRS-1 protein.
    Purification
    Immunoaffinity Chromatography using epitope-specific immunogen.
    Immunogen
    The antiserum was produced against synthesized non-phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 636 (P-M-SP-P-K).
  • Application Notes
    Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody (in aliquots) at -20 °C.
  • Target
    IRS1
    Alternative Name
    IRS1 (IRS1 Antibody Abstract)
    Synonyms
    HIRS-1, G972R, IRS-1, IRS1IRM, irs1, irsu, IRS1, irs-1, irs1-a, insulin receptor substrate 1, insulin receptor substrate 2, insulin receptor substrate 1 S homeolog, IRS1, Irs1, irs2, irs1.S
    Background
    Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed.Synonyms: IRS-1, Insulin receptor substrate 1
    Gene ID
    3667
    NCBI Accession
    NP_005535
    UniProt
    P35568
    Pathways
    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
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