MARCKS antibody (Ser158)

Catalog No. ABIN197560

This Rabbit Polyclonal antibody specifically detects MARCKS in WB and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MARCKS antibody (Ser158) (ABIN197560)

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCKS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-MARCKS Antibody

Binding Specificity: Ser158

Specificity: This antibody detects endogenous levels of total MARCKS protein.

Purification: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human MARCKS around the phosphorylation site of serine 158 (R-F-Sp-F-K).

Application Details

Application Notes: Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: MARCKS

Background: MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL

Gene ID: 4082

NCBI Accession: NP_002347

UniProt: P29966