NFKBIE antibody (Ser22)

Catalog No. ABIN197570

The Rabbit Polyclonal anti-NFKBIE antibody is suitable to detect NFKBIE in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for NFKBIE antibody (Ser22) (ABIN197570)

Target: NFKBIE (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NFKBIE Antibody

Binding Specificity: Ser22

Specificity: IkB-ε antibody detects endogenous levels of total IkB-ε protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human IkB-epsilon around the phosphorylation site of serine 22 ( I-E-SP-L-R).

Application Details

Application Notes: Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for NFKBIE

Target: NFKBIE (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))

Alternative Name: NFKBIE / IKBE

Background: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine). For some genes, activation requires NFKB interaction with other transcription factors, such as STAT, AP1 (JUN), and NFAT.Synonyms: I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilon, NF-kappa-B inhibitor epsilon

Gene ID: 4794

NCBI Accession: NP_004547

UniProt: O00221

Pathways: NF-kappaB Signaling, Maintenance of Protein Location