Sonic Hedgehog antibody (AA 199-437)

Catalog No. ABIN6757522

This anti-Sonic Hedgehog antibody is a Rat Monoclonal antibody detecting Sonic Hedgehog in WB and ELISA. Suitable for Mouse.

Quick Overview for Sonic Hedgehog antibody (AA 199-437) (ABIN6757522)

Target: Sonic Hedgehog (SHH)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: AA 199-437

Specificity: Recognizes mouse Shh C-terminal peptide. In direct ELISA, does not cross-react with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide or rmShh N-terminal peptide.

Purification: Protein G purified

Immunogen: Purified, E. coli-derived, recombinant mouse Sonic Hedgehog C-terminal peptide (rmShh C-terminal peptide, aa 199-437).

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and ELISA. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for rmShh C-terminal peptide is approximately 1 ng/well. Western Blot: 1-2 μg/mL with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for recombinant mouse Shh C-terminal peptide is approximately 0.5 ng/lane under non-reducing and reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: Sonic Hedgehog / SHH

Background: Name/Gene ID: SHH
Subfamily: Cysteine C46
Family: Protease

Synonyms: SHH, HHG-1, HLP3, Sonic hedgehog, Sonic hedgehog homolog, MCOPCB5, Sonic hedgehog protein, HHG1, HPE3, SMMCI, TPT, TPTPS

Gene ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development