SIRPA antibody

Catalog No. ABIN198026

The Mouse Monoclonal anti-SIRPA antibody has been validated for FACS, IHC, IHC (p) and IHC (fro). It is suitable to detect SIRPA in samples from Rat.

Quick Overview for SIRPA antibody (ABIN198026)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SIRPA Antibody

Specificity: Recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. Binds to a different epitope on SIRP than ED9. Does not block the interaction of CD172a-CD47.

Purification: Protein G purified

Immunogen: Rat peritoneal macrophages.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:100 - 1:200), IHC, IHC-Fr, IHC-P

Usage: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen and paraffin). Flow Cytometry: 1:100-1:200. Use 10 μL to label 1 x10⁶ in 100 μL. Immunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e. g. trypsin or pronase.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172A / SIRPA

Background: Name/Gene ID: SIRPA

Synonyms: SIRPA, CD172A, CD172a antigen, Inhibitory receptors shps-1, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, p84, SHPS-1, SHPS1, SIRP, Sirp-alpha-1, Sirp-alpha-3, Sirp-alpha1, MYD1, SIRPalpha, SIRPalpha2, BIT, MFR, MYD-1, PTPNS1, SHP substrate 1, Sirp-alpha-2

Gene ID: 140885

UniProt: P78324