CD161 antibody

Catalog No. ABIN1981864

This Mouse Monoclonal antibody specifically detects CD161 in FACS, WB and IHC (fro). It exhibits reactivity toward Human and Non-Human Primate and has been mentioned in 4+ publications.

Quick Overview for CD161 antibody (ABIN1981864)

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD161 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HP-3G10

Product Details anti-CD161 Antibody

Purpose: Anti-Hu CD161 Purified

Specificity: The mouse monoclonal antibody HP-3G10 recognizes an extracellular epitope of CD161, a type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: human NK cells

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Do not use after expiration date stamped on vial label.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD161 antibody (ABIN1981864)

Tresoldi, DellAlbani, Stabilini, Jofra, Valle, Gagliani, Bondanza, Roncarolo, Battaglia: "Stability of human rapamycin-expanded CD4+CD25+ T regulatory cells." in: Haematologica, Vol. 96, Issue 9, pp. 1357-65, (2011) (PubMed).

Germain, Meier, Jensen, Knapnougel, Poupon, Lazzari, Neisig, Håkansson, Dong, Wagtmann, Galsgaard, Spee, Braud: "Induction of lectin-like transcript 1 (LLT1) protein cell surface expression by pathogens and interferon-? contributes to modulate immune responses." in: The Journal of biological chemistry, Vol. 286, Issue 44, pp. 37964-75, (2011) (PubMed).

Goetzl, Huang, Kon, Patel, Schwartz, Fast, Ferrucci, Madara, Taub, Longo: "Gender specificity of altered human immune cytokine profiles in aging." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 9, pp. 3580-9, (2010) (PubMed).

Pozo, Valés-Gómez, Mavaddat, Williamson, Chisholm, Reyburn: "CD161 (human NKR-P1A) signaling in NK cells involves the activation of acid sphingomyelinase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 4, pp. 2397-406, (2006) (PubMed).

Target Details for CD161

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Alternative Name: CD161

Background: Killer cell lectin like receptor B1,CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.,NKRP1A, KLRB1, CLEC5B

Gene ID: 3820

UniProt: Q12918