CCR7 antibody

Catalog No. ABIN1981865

There are 2+ publications for this product available. The Rat Monoclonal anti-CCR7 antibody is suitable to detect CCR7 in samples from Mouse. It has been validated for FACS and IP.

Quick Overview for CCR7 antibody (ABIN1981865)

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CCR7 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 4B12

Product Details anti-CCR7 Antibody

Purpose: Anti-Ms CD197 Purified

Cross-Reactivity (Details): Mouse

Characteristics: The rat monoclonal antibody 4B12 recognizes an extracellular epitope of murine CD197 / CCR7 (chemokine receptor 7), a 43 kDa G-protein-coupled receptor for chemokines CCL19 and CCL21.

Purification: Purified by protein-G affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Murine CD197-transfected RBL-2H3 cells

Isotype: IgG2a, kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CCR7 antibody (ABIN1981865)

Eisenbarth, Williams, Colegio, Meng, Strowig, Rongvaux, Henao-Mejia, Thaiss, Joly, Gonzalez, Xu, Zenewicz, Haberman, Elinav, Kleinstein, Sutterwala, Flavell: "NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells." in: Nature, Vol. 484, Issue 7395, pp. 510-3, (2012) (PubMed).

Huggins, Paschalidis, Flower, Perretti, DAcquisto: "Annexin-1-deficient dendritic cells acquire a mature phenotype during differentiation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 4, pp. 985-96, (2009) (PubMed).

Target Details for CCR7

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Alternative Name: CD197

Background: Chemokine (C-C motif) receptor 7,CD197 / CCR7 (chemokine receptor 7) is a seven membrane-spanning protein serving as a receptor for chemokines CCL19 and CCL21. It is expressed on most naive T cells, some hematopoietic stem cells, mature dendritic cells, NK cells and some memory T cells and B cells subsets. CD197 plays important roles in development of thymocytes, and regulating the recirculation and homing of lymphocytes and dendritic cells to secondary lymphoid organs.,CCR7, CC-CKR-7, EBI1

Gene ID: 12775

UniProt: P47774

Pathways: Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process