CD247 antibody (pTyr153)

Catalog No. ABIN1981867

This Mouse Monoclonal antibody specifically detects CD247 in WB and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for CD247 antibody (pTyr153) (ABIN1981867)

Target: CD247 (CD247 Molecule (CD247))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD247 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: EM-17

Product Details anti-CD247 Antibody

Binding Specificity: pTyr153

Purpose: Anti-Hu CD3 zeta (pY153) Purified

Specificity: The mouse monoclonal antibody EM-17 recognizes phosphorylated intracellular tyrosine 153 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.

Cross-Reactivity (Details): Human, Mouse

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: A phospho specific peptide corresponding to the amino acids surrounding tyrosine 153 of mouse CD3 zeta linked to KLH

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 2 - 5 μg/mL, positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended.
Flow cytometry: Intracellular staining, recommended dilution: 1-4 μg/mL, positive control: Jurkat cells treated with pervanadate.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Do not use after expiration date stamped on vial label.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD247

Target: CD247 (CD247 Molecule (CD247))

Alternative Name: CD3 zeta

Background: CD247 Molecule,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 zeta chain, CD247, T3Z, TCRz, IMD25

Gene ID: 919

UniProt: P20963

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway