LRRC32 antibody

Catalog No. ABIN1981876

This Mouse Monoclonal antibody specifically detects LRRC32 in WB and FACS. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for LRRC32 antibody (ABIN1981876)

Target: LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LRRC32 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: GARP5

Product Details anti-LRRC32 Antibody

Purpose: Anti-Hu GARP Purified

Specificity: The mouse monoclonal antibody GARP5 recognizes GARP / LRRC32, an approximately 80 kDa glycoprotein expressed e.g. on the surface of megakaryocytes, platelets and activated Treg cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified human sGARP protein

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 1-2 μg/mL, positive control: human thrombocytes, reducing conditions

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Do not use after expiration date stamped on vial label.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-LRRC32 antibody (ABIN1981876)

Wang, Zhu, Dong, Shi, Lu, Springer: "GARP regulates the bioavailability and activation of TGF?." in: Molecular biology of the cell, Vol. 23, Issue 6, pp. 1129-39, (2012) (PubMed).

Target Details for LRRC32

Target: LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

Alternative Name: GARP

Background: Leucine rich repeat containing 32,GARP (glycoprotein A repetitions predominant protein), also known as garpin or LRRC32 (leucin-rich repeat containing protein 32) is an approximately 80 kDa transmembrane glycoprotein detected on the surface of megakaryocytes, platelets, and activated Treg cells. It binds to the latency-associated peptide (LAP) domain of pro-TGF beta and regulates its storage and activation. The expression of GARP on Treg cells seems to be necessary for their suppressive functions.,GARP, D11S833E

Gene ID: 2615

UniProt: Q14392

Pathways: Activated T Cell Proliferation