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CD300E antibody (APC)

CD300E Reactivity: Human FACS Host: Mouse Monoclonal UP-H2 APC
Catalog No. ABIN1981879
  • Target See all CD300E Antibodies
    CD300E (CD300e Molecule (CD300E))
    Reactivity
    • 34
    • 8
    Human
    Host
    • 22
    • 12
    Mouse
    Clonality
    • 23
    • 11
    Monoclonal
    Conjugate
    • 9
    • 5
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD300E antibody is conjugated to APC
    Application
    • 20
    • 14
    • 11
    • 5
    • 4
    • 3
    • 1
    Flow Cytometry (FACS)
    Specificity
    The mouse monoclonal antibody UP-H2 recognizes an extracellular epitope on CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.
    Cross-Reactivity (Details)
    Human
    Purification
    Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    CD300e-HA-transfected cells
    Clone
    UP-H2
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CD300E Primary Antibody
  • Application Notes
    Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
    Comment

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze. Avoid prolonged exposure to light.
    Do not use after expiration date stamped on vial label.
    Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Brckalo, Calzetti, Pérez-Cabezas, Borrs, Cassatella, López-Botet: "Functional analysis of the CD300e receptor in human monocytes and myeloid dendritic cells." in: European journal of immunology, Vol. 40, Issue 3, pp. 722-32, (2010) (PubMed).

    Aguilar, Alvarez-Errico, García-Montero, Orfao, Sayós, López-Botet: "Molecular characterization of a novel immune receptor restricted to the monocytic lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 11, pp. 6703-11, (2004) (PubMed).

  • Target
    CD300E (CD300e Molecule (CD300E))
    Alternative Name
    CD300e (CD300E Products)
    Synonyms
    BC034097 antibody, CLM2 antibody, Cd300le antibody, TREM5 antibody, CD300LE antibody, CLM-2 antibody, CMRF35-A5 antibody, IREM-2 antibody, IREM2 antibody, PIgR-2 antibody, PIgR2 antibody, CD300E molecule antibody, CD300e molecule antibody, Cd300e antibody, CD300E antibody
    Background
    CD300e molecule,CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.,CLM2, CMRF35-A5, LMIR6, IREM-2, PlgR2
    Gene ID
    342510
    UniProt
    Q496F6
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