UBE2N antibody

Catalog No. ABIN198227

The Mouse Monoclonal anti-UBE2N antibody has been validated for WB, ELISA and IF. It is suitable to detect UBE2N in samples from Human, Rat and Mouse.

Quick Overview for UBE2N antibody (ABIN198227)

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This UBE2N antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-UBE2N Antibody

Specificity: Specific for the ~21kD Ubc13 protein. Reactivity has been confirmed with human HepG2 cell lysates, mouse NIH 3T3 cells, and rat Rat2 fibroblasts.

Purification: Protein A purified

Immunogen: Recombinant human Ubc13.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (1 - 5 μg/mL), WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot and ELISA. Immunofluorescence: 1-5 μg/mL. Western Blot: 1-3 μg/mL. ELISA: 0.1-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for UBE2N

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Alternative Name: UBE2N / UBC13

Background: Name/Gene ID: UBE2N

Synonyms: UBE2N, BLU, UbcH13, UbcH-ben, Ubiquitin carrier protein N, Yeast UBC13 homolog, UBC13, Ubiquitin-protein ligase N

Gene ID: 7334

UniProt: P61088

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway