CTNNA1 antibody (C-Term)

Catalog No. ABIN198449

The Mouse Monoclonal anti-CTNNA1 antibody has been validated for WB, IHC, ELISA, IF, IP and IHC (p). It is suitable to detect CTNNA1 in samples from Human, Mouse, Rat, Chicken and Xenopus laevis.

Quick Overview for CTNNA1 antibody (C-Term) (ABIN198449)

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Reactivity: Human, Mouse, Rat, Chicken, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNNA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7A4

Product Details anti-CTNNA1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes mouse a-catenin. Does not cross-react with the related beta- and gamma-catenin proteins. Species cross-reactivity: Human, rat, chicken, Xenopus. Lysates Tested: HeLa cells, A431 cells, WI-38 human fibroblasts, Xenopus cytosol, NIH 3T3 cells.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminus of mouse a-catenin.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (2 - 5 μg/mL), IHC, IHC-P, IP, WB (1 μg/mL)

Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 2-5 μg/mL. ELISA: 0.1-1 μg/mL. Western Blot: 1 μg/mL. Immunohistochemistry ( formalin- fixed, paraffin-embedded tissue): 2-10 μg/mL. Heat-Induced Epitope Retrieval (HIER) is required. Expected staining pattern: Membrane with limited cytoplasmic. Immunoprecipitation: 5 μg. Positive control: Pancreas/breast tumor/heart.Tested with Xenopus samples using Western blot only.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CTNNA1

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Alternative Name: CTNNA1

Background: Name/Gene ID: CTNNA1
Family: Catenin

Synonyms: CTNNA1, AlphaE-catenin, Alpha-E-catenin, Alpha(E)-catenin, Alpha-1 catenin, Alpha-catenin, Cadherin-associated protein, Alpha E-catenin, CAP102, Catenin alpha-1

Gene ID: 1495

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location