MCAM antibody

Catalog No. ABIN198956

The Mouse Monoclonal anti-MCAM antibody is suitable to detect MCAM in samples from Human. It has been validated for IHC, FACS, WB, ELISA and IHC (fro).

Quick Overview for MCAM antibody (ABIN198956)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCAM antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MCAM Antibody

Specificity: Reacts with the human MUC18 (CD146, Mel-CAM, MCAM) protein. Reactivity is confirmed with human SK-Mel-37 melanoma cell lysates.

Purification: Protein A purified

Immunogen: Recombinant human MUC18 (D1-D5) Fc protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), Flo (5 - 10 μg/mL), IHC, IHC-Fr, WB (1 - 10 μg/mL)

Usage: Suitable for use in Immunohistochemistry, Flow Cytometry, ELISA and Western Blot. Immunohistochemistry (frozen): 5-10 μg/mL. Flow Cytometry: 5-10 μg/mL. ELISA: 0.1-1 μg/mL. Western Blot: 1-10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: MCAM / CD146

Background: Name/Gene ID: MCAM
Family: Immunoglobulin

Synonyms: MCAM, CD146, CD146 antigen, Gicerin, Melanoma adhesion molecule, MUC18, p1h12

Gene ID: 4162

UniProt: P43121