GRP94 antibody (N-Term)

Catalog No. ABIN199196

The Rabbit Polyclonal anti-GRP94 antibody has been validated for WB, ELISA and IP. It is suitable to detect GRP94 in samples from Human, Mouse and Rat.

Quick Overview for GRP94 antibody (N-Term) (ABIN199196)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-GRP94 Antibody

Binding Specificity: N-Term

Specificity: Recognizes mouse, rat and human gp96 proteins. Reactivity has been confirmed with mouse and rat brain homogenates by Western Blot and immunoprecipitation, and with formalin-fixed, paraffin-embedded (FFPE) human tonsil tissue by immunohistochemistry.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the N-terminal region of the mouse and human gp96 protein. Species sequence Homology: Pig, cow, and dog: 100%.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IP (1 - 5 μg/mL), WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL. Identifies a single band at ~96kD. Immunoprecipitation: 1-5 μg/mL. Immunohistochemistry: 0.5-1 μg/mL Heat induced epitope retrieval (HIER) with EDTA,  pH 8.0, is required prior to staining for formalin-fixed, paraffin-embedded (FFPE) tissues.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: HSP90B1 / GP96 / GRP94

Background: Name/Gene ID: HSP90B1

Synonyms: HSP90B1, ECGP, Endoplasmin, gp96 homolog, gp96, GRP-94, GRP94, Tumor rejection antigen 1, TRA1

Gene ID: 7184

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades