ZBTB33 antibody (N-Term)

Catalog No. ABIN199363

This Mouse Monoclonal antibody specifically detects ZBTB33 in WB, ELISA, IP and IF. It exhibits reactivity toward Human, Mouse, Rat, Dog and Chicken.

Quick Overview for ZBTB33 antibody (N-Term) (ABIN199363)

Target: ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZBTB33 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-ZBTB33 Antibody

Binding Specificity: N-Term

Specificity: Reacts with the ~95kD Kaiso protein. Reactivity is confirmed with mouse mammary adenocarcinoma cell line CSML0. Reactivity has been confirmed in MDCK (dog), NBTII (rat), HCT116 (human) and chicken embryo fibroblasts.

Purification: Protein A purified

Immunogen: Fusion protein encompassing the N-terminus of mouse Kaiso.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (10 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Immunofluorescence, Western Blot and ELISA. Immunoprecipitation: 10 μg/IP reaction. Immunofluorescence: 10 μg/mL. Western Blot: 1-3 μg/mL. ELISA: 0.1-1 μg/mL. Note: Immunofluorescent assays were performed on cell lines fixed with 100 % methanol at -20 ?C for 7 minutes.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for ZBTB33

Target: ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))

Alternative Name: ZBTB33 / Kaiso

Background: Name/Gene ID: ZBTB33

Synonyms: ZBTB33, Kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, Kaiso transcription factor, ZNF348

Gene ID: 10009

UniProt: Q86T24