LHX1 antibody (C-Term)

Catalog No. ABIN199380

This anti-LHX1 antibody is a Rabbit Polyclonal antibody detecting LHX1 in WB, IHC, IHC (p), ICC and IP. Suitable for Human, Mouse, Rat and Frog.

Quick Overview for LHX1 antibody (C-Term) (ABIN199380)

Target: LHX1 (LIM Homeobox 1 (LHX1))

Reactivity: Human, Mouse, Rat, Frog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LHX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-LHX1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes frog LIM-1. Does not appear to cross-react with LIM-5 (the closest homolog of LIM-1 in frog) on tissue sections, but does cross-react with LIM-5 in Western Blot and Immunoprecipitation. Species cross-reactivity: human, rat, mouse and fish. Also reacts with LIM-1 protein from whole-mount fish embryo.

Purification: Protein A purified

Immunogen: C-terminal portion of frog LIM-1 protein.

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:500), IHC, IHC-P (1:200 - 1:1000), IP (1:200), WB (1:3000 - 1:6000)

Usage: Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Immunoprecipitation. Not suitable for Immunofluorescence. Immunohistochemistry (paraffin embedded sections): 1:200-1:1000 (Fish and whole mounts: 1:500) Recommended fixative: MEMFA. Western Blot: 1:3000-1:6000. Immunocytochemistry: 1:500 on P19 cell line. Immunoprecipitation: 1:200.

Comment:

Target Species of Antibody: Frog

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02M PBS,  pH 7.6, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for LHX1

Target: LHX1 (LIM Homeobox 1 (LHX1))

Alternative Name: LHX1

Background: Name/Gene ID: LHX1

Synonyms: LHX1, Homeobox protein Lim-1, LIM1, HLim-1, LIM homeobox 1, LIM homeobox protein 1, LIM-1, LIM/homeobox protein Lhx1

Gene ID: 3975

UniProt: P48742