CTNNB1 antibody (C-Term)

Catalog No. ABIN200360

The Mouse Monoclonal anti-CTNNB1 antibody has been validated for WB, IHC, IF, IHC (p) and IP. It is suitable to detect CTNNB1 in samples from Human, Mouse and Chicken.

Quick Overview for CTNNB1 antibody (C-Term) (ABIN200360)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reactivity: Human, Mouse, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNNB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-CTNNB1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes chicken b-Catenin protein (~92kD). Does not cross-react with the related 83kD g-catenin (plakoglobin) protein. Also specifically detects an additional 120kD protein of unknown identity in extracts derived from A431 cells. This band is not detected in lysates derived from HeLa cells or human fibroblasts. Species cross-reactivity: Human & mouse. Reactivity with rat is likely but not confirmed.

Purification: Protein A purified

Immunogen: Fusion protein consisting of the maltose binding protein fused to a 100 amino acid segment of the C-terminus of chicken b-Catenin.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IF (5 - 10 μg/mL), IHC, IHC-P, IP, WB (1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 5-10 μg/mL. Western Blot: 1 μg/mL. Immunoprecipitation: 2-5 μg. Immunohistochemistry (Formalin-fixed and paraffin-embedded tissue): 2-10 μg/mL. Requires heat induced epitope retrieval. Positive control: HeLa, A431 and WI-38 human cell lines.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Alternative Name: CTNNB1 / Beta Catenin

Background: Name/Gene ID: CTNNB1
Family: Catenin

Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin

Gene ID: 1499

UniProt: P35222

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2