GJB1 antibody (Cytoplasmic Domain)

Catalog No. ABIN200656

This Mouse Monoclonal antibody specifically detects GJB1 in WB, IHC, IF, ELISA and IHC (fro). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for GJB1 antibody (Cytoplasmic Domain) (ABIN200656)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GJB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GJB1 Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: Recognizes rat Connexin-32. Species cross-reactivity: human and mouse.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-32.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (5 - 10 μg/mL), IHC, IHC-Fr, WB (1 μg/mL)

Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1.0 μg/mL. Western Blot: 1 μg/mL. Reactivity has been confirmed by Western blot analysis of extracts derived from mouse liver, rat liver, rat primary hepatocytes and rat spinal cord. Immunohistochemistry (frozen sections): 1-2 μg/mL. Immunofluorescence: 5-10 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: GJB1 / CX32 / Connexin 32

Background: Name/Gene ID: GJB1
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJB1, Connexin 32, CMTX1, Connexin-32, CX32, Gap junction beta-1 protein, Gap junction protein beta-1, CMTX

Gene ID: 2705

UniProt: P08034

Pathways: Cell-Cell Junction Organization