CREB1 antibody (pSer133)

Catalog No. ABIN200678

The Mouse Monoclonal anti-CREB1 antibody has been validated for WB. It is suitable to detect CREB1 in samples from Human, Mouse and Rat.

Quick Overview for CREB1 antibody (pSer133) (ABIN200678)

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CREB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CREB1 Antibody

Binding Specificity: AA 125-135, pSer133

Specificity: Recognizes phosphorylated CREB at 46kD. May also recognize p30 and p38 proteins, which likely correspond to phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology with the phosphorylated CREB immunizing peptide. Species cross-reactivity: Human and mouse. Rat Cross-reactivity predicted based on sequence homology.

Purification: Gel filtration

Immunogen: KLH-conjugated, synthetic peptide (REILSRRP(pS)YR) corresponding to amino acids 125-135 of human CREB. The immunizing sequence is identical in human and mouse.

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 0.5-2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as frozen serum-free cell culture supernatant in 30 μL of 2X PBS containing PEG 3000, sucrose, and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Alternative Name: CREB1 / CREB

Background: Name/Gene ID: CREB1
Family: Transcription factor

Synonyms: CREB1, CREB-1, Transactivator protein, CREB

Gene ID: 1385

UniProt: P16220

Pathways: TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation