Histone H2B antibody (acLys12, acLys15, acLys20)

Catalog No. ABIN201041

The Rabbit Polyclonal anti-Histone H2B antibody has been validated for WB. It is suitable to detect Histone H2B in samples from Human, Mouse, Rat and Cow.

Quick Overview for Histone H2B antibody (acLys12, acLys15, acLys20) (ABIN201041)

Target: Histone H2B

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Histone H2B Antibody

Binding Specificity: acLys12, acLys15, acLys20

Specificity: Recognizes acetylated Histone H2B, Mr ~15kD. Does not recognize acetylated Histone H2A or unacetylated recombinant Histone H2B protein. Species cross-reactivity: Human. Predicted to cross-react with mouse, rat and bovine based on sequence homology.

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide (. S[AcK]SAPAPK[AcK]GS[AcK]KAIT[AcK]AQ.) in which AcK corresponds to acetyl-lysine at residues 5, 12, 15 and 20 of human Histone H2B. The immunizing sequence has 24 out of 26 identical amino acids in mouse, rat and bovine.

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: Suitable for use in Western Blot. Western Blot Analysis: 1:40000-1:80000 dilution of this lot detected acetylated Histone H2B in sodium butyrate treated, acid extracted proteins from HeLa cells. Unacetylated recombinant Histone H2B was not detected.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Histone H2B

Target: Histone H2B